A novel method has been developed for the speciation of chromium in natural water samples based on cloud point extraction (CPE) separation and preconcentration, and determination by graphite furnace atomic absorption spectrometry (GFAAS). In this method, Cr(III) reacts with 1phenyl3methyl4benzoylpyrazol5one (PMBP) yielding a hydrophobic complex, which then is entrapped in the .